M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation

Bacteriophage - Activated Superparamagnetic Beads for Affinity Separation

Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody

Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...

Initiation and termination signals for transcription in bacteriophage M13.

Transcription of the infrequently expressed phage M13 genome domain, comprising genes III, VI, I and IV, has been studied in detail by hybridization and S1-nuclease mapping studies. The contiguous genes III and VI are transcribed via an 1800 nucleotide-long RNA molecule that is initiated at a promoter which overlaps with the Rho-independent termination signal between genes III and VIII. Its syn...

Microfluidic Separation System for Magnetic Beads

Lab-on-a-chip technologies and the μTAS have gained importance in recent years. The integration of all functions and components needed for the analysis of a sample in a microfluidic system has to be achieved. Magnetic materials are widely used in these systems for various applications such as magnetohydrodynamic pumps and valves. Especially, in terms of separation and detection of biological sa...

From the Cover: Single M13 bacteriophage tethering and stretching

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